Knipscheer: DNA repair

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The Knipscheer group studies molecular mechanisms of DNA replication and repair.

Cellular processes that maintain the integrity of our genome are crucial to prevent genetic diseases such as cancer. The main interest of our laboratory is to decipher the molecular details of these processes. Currently, we focus on understanding how toxic DNA lesions, called interstrand crosslinks (ICLs), are repaired, and how stable secondary DNA structures (G-quadruplexes) are resolved. We study these processes in the context of active DNA replication and make use of Xenopus egg extracts that support highly regulated vertebrate DNA replication in vitro. In this experimental system, we can recapitulate both ICL repair and G-quadruplex replication in a test tube, allowing us to gain insights in their molecular mechanisms.

Key publications

Dev Biol. 428(2):300-309

Download|2017

EMBO J. 36(14):2034-2046

Download|2017

FANCJ promotes DNA synthesis through G-quadruplex structures.

Castillo Bosch P, Segura-Bayona S, Koole W, van Heteren J, Dewar J, Tijsterman M#, Knipscheer P#

EMBO J. 33(21):2521-33

Download|2014

XPF-ERCC1 acts in unhooking DNA interstrand crosslinks in cooperation with FANCD2 and FANCP/SLX4.

Klein Douwel D*, Boonen RACM*, Long DT, Szypowska AA, Räschle M, Walter JC, Knipscheer P

Mol Cell. 54(3):460-71

Download|2014

The Fanconi anemia pathway promotes replication-dependent DNA interstrand crosslink repair.

Knipscheer P, Räschle M, Smogorzewska A, Enoiu M, Ho TV, Schärer OD, Elledge SJ, Walter JC

Science. 326:1698-1701

Download|2009

Mechanism of replication-coupled DNA interstrand crosslink repair.

Räschle M, Knipscheer P, Enoiu M, Angelov T, Sun J, Griffith JD, Ellenberger TE, Schärer OD, Walter JC

Cell. 134:969-980

Download|2008

Group leader

Puck Knipscheer

Puck Knipscheer is group leader at the Hubrecht Institute. Her group studies how toxic DNA lesions, called DNA interstrand crosslinks (ICLs), are repaired and how secondary DNA structures (G-quadruplexes) are resolved. These processes are important to maintain the integrity of our genome which is crucial to prevent genetic diseases such as cancer.  The Knipscheer group studies these processes during active DNA replication in Xenopus egg extracts. These extracts recapitulate both processes under physiological conditions, which enables them to study the molecular details of these important pathways.

Scientific training and positions

Awards


Group members

Puck Knipscheer

Principal Investigator

Merlijn Witte

Technician

Nerea Martin-Pintado Zugasti

Postdoc

Koichi Sato

Postdoc

Wouter Hoogenboom

PhD Student

Alice Bolner

PhD Student

Roxanne van der Sluijs

PhD Student

Merijn Kerstens

Student

Vera Janssen

Student

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