Rabouille: Cellular stress in Drosophila

Back to research groups

The Rabouille group studies the cell biology of stress in Drosophila.

Delivery of newly synthesized proteins to the plasma membrane and the extracellular medium takes place in the membrane organelles of the secretory pathway. We focus on the ER exit sites (ERES), that are characterized by the machinery mediating COPII coat vesicle budding. This includes the large scaffold ERES protein Sec16 that optimizes COPII vesicle dynamics. This molecular machinery is tightly controlled and is regulated by signaling. In this context, we have studied the role of ERK7 in Drosophila S2 cells in response to serum starvation, and have shown that amino-acid starvation leads to the formation of a of novel membrane-less stress assembly, the Sec bodies, as well as stress granules.

Key publications

Modulation of the secretory pathway by amino-acid starvation

van Leeuwen W, van der Krift F, Rabouille C.

Journal of Cell Biology

Download|2018

Phospho-Rasputin stabilization by Sec16 is required for stress granules formation upon amino-acid starvation

Aguilera-Gomez A, Zacharogianni M, van Oorschot MM, Genau H, Veenendaal T, Sinsimer KS, Gavis ER, Behrends C and Rabouille C.

Cell Reports 20:935-948

Download|2017

Dev Biol “Hubrecht Institute centennial, from embryos to stem cells” 428(2):310-317

Download|2017

Curr Opi Cell Biol. 47:34-42

Download|2017

In vivo vizualisation of mono-ADP-ribosylation by dPARP16 upon amino-acid starvation

Aguilera-Gomez A, Van Oorschot MM, Veenendaal T and Rabouille C.

Elife 3: e21475

Download|2016

Trends Cell Biol. 27:230-240

Download|2016

TORC2 mediates the heat stress response in Drosophila by promoting the formation of stress granules

Jevtov I*, Zacharogianni M*, van Oorschot MM*, Aguilera Gomez A, Van Hazelhoff G, Vuiliez I, Braakman I, Hafen E, Stocker H, Rabouille C.**

J Cell Sci 128: 2497-2508

Download|2015

A stress assembly that confers cell viability by preserving ERES components during amino-acid starvation

Zacharogianni M, Aguilera-Gomez A, Smout J, Veenendaal T, van Oorschot MM, Rabouille C.

Elife 3: e04132

Download|2014

Hum Mol Genet 23:5961-75

Download|2014

GRASP65 controls the cis Golgi integrity in vivo

Veenendaal T, Jarvela T, Grieve A, van Es J, Linstedt A, Rabouille C.

Biology Open 3:431-43

Download|2014

Drosophila patterning is established by differential association of mRNAs with P bodies

Weil TT, Parton RM, Herpers B, Soetaert J, Veenendaal T, Xanthakis D, Dobbie IM, Halstead J, Hayashi R, Rabouille C*, Davis I*

Nat. Cell Biol. 14:1305-13

Download|2012

ERK7 is a negative regulator of protein secretion in response to amino acid starvation by modulating Sec16 membrane association

Zacharogianni M, Kondylis V, Tang Y, Xanthakis D, Farhan H, Fuchs F, Boutros M, Rabouille C.

EMBO J. 30:3684-700

Download|2011

Group leader

Catherine Rabouille

Catherine Rabouille is group leader at the Hubrecht Institute and Professor of Cell Biology at the University Medical Center Groningen, and is also affiliated with the University Medical Center Utrecht. After studying the early secretory pathway in Drosophila melanogaster, her group now focuses on how nutrient stress (serum and amino-acid starvation) regulates the early secretory pathway and leads to the formation of membrane-less stress assemblies.

Scientific training and positions

Awards

Current other activities


Group members

Catherine Rabouille

Principal Investigator

Rianne Grond

Technician

Wessel van Leeuwen

PhD Student

Chujun Zhang

PhD Student

Felix van der Krift

Student

Apfrida Kendek

Student

We welcome motivated undergraduate students to participate in our projects. Besides learning many cell biology techniques (tissue culture, Westerns, generating DNA constructs, transfections) as well as fly genetics, students can get familiar with imaging techniques depending on their own interests and experience.