den Hertog: Protein-tyrosine phosphatases in development

Back to research groups

The Den Hertog group studies the function of protein-tyrosine phosphatases in development and disease, using biochemical and cell biological approaches.

Protein phosphorylation on tyrosine residues is an important cell signaling mechanism that has a pivotal role in many biological processes, including cell proliferation, migration and transformation. Cellular protein phosphotyrosine levels are regulated by the antagonistic activities of the protein-tyrosine kinases (PTKs) and protein-tyrosine phosphatases (PTPs).

Key publicationsView all publications

Blood 123, 184-190

Download|2014

Noonan and LEOPARD syndrome Shp2 variants induce heart displacement defects in zebrafish.

Bonetti M, Paardekooper Overman J, Tessadori F, Noël E, Bakkers J and den Hertog J

Development 141, 1961-1970

Download|2014

EMBO J. 21: 493-503.

Download|2002

Receptor protein-tyrosine phosphatase alpha activates pp60c-src and is involved in neuronal differentiation.

den Hertog J, Pals CEGM, Peppelenbosch MP, Tertoolen LGJ, de Laat SW, Kruijer W

EMBO J. 12: 3789-3798.

Download|1993

Other publications

Regeneration 4, 217-226

Download|2017

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

Ciarlo C, Kaufman CK, Michael J, D’Amato C, Kinikoglu B, Yang S, Blokzijl-Franke S, den Hertog J, Schlaeger T, Zhou Y, Liao EC, Zon LI

eLife 6, e29145

Download|2017

Dev Biol 428, 259-260

Download|2017

Dev Biol 428, 261-263

Download|2017

Adv Exp Med Biol 916, 87-101

Download|2016

Methods Mol Biol. 1447, 351-372

Download|2016

Comparative interactomics analysis of different ALS-associated proteins identifies converging molecular pathways

Blokhuis AM, Koppers M, Groen EJN, Dini Modigliani S, van den Heuvel DMA, Anink JJ, Fumoto K, van Diggelen F, Snelting A, Sodaar P, Demmers JAA, Veldink JH, Aronica E, Bozzoni I, den Hertog J, van den Berg LH, Pasterkamp RJ

Acta Neurpath 132, 175-196

Download|2016

PLoS One 11, e0154771

Download|2016

Comparative interactomics analysis of different ALS-associated proteins identifies converging molecular pathways

Blokhuis AM, Koppers M, Groen EJN, Dini Modigliani S, van den Heuvel DMA, ... den Hertog J, van den Berg LH, Pasterkamp RJ

Acta Neurpath 132, 175-196

2016

Receptor protein tyrosine phosphatase alpha enhances rheumatoid synovial fibroblast signaling and promotes arthritis in mice

Stanford SM, Svensson MN, Sacchetti C, Pilo CA, Wu DJ, Kiosses WB, ... den Hertog J, Elson A, Sap J, Mydel P, Boyle DL, Corr M, Firestein GS, Bottini N.

Arthritis Rheumatol 68, 359-369

Download|2016

Pten function in zebrafish: Anything but a fish story

Stumpf M, Choorapoikayil S and den Hertog J

Methods, 77-78, 191-196

Download|2015

Heterozygous germline mutations in A2ML1 are associated with a disorder clinically related to Noonan syndrome

Vissers LELM, Bonetti M, Paardekooper Overman J, Nillesen WM ... Tartaglia M, van der Burgt I, Yntema HG and den Hertog J

Eur J Hum Gen, 23, 317-324

Download|2015

Phosphoproteomics-mediated identification of Fer kinase as a target of mutant Shp2 in Noonan and LEOPARD syndrome

Paardekooper Overman J, Preisinger C, Prummel K, Bonetti M, Giansanti P, Heck A and den Hertog J

PLoS ONE 9, e106682

Download|2014

A Unified Nomenclature and Amino Acid Numbering for Human PTEN

Pulido R, Baker SJ, Barata JT, Carracedo A, Cid VJ, Chin-Sang ID, Davé V, den Hertog J ... Woscholski R, Wu H, Leslie NR

Sci Signal 7, pe15

Download|2014

PZR coordinates Noonan and LEOPARD syndrome signaling in zebrafish and mice

Paardekooper Overman J, Yi JS, Bonetti M, Soulsby M, Preisinger C, Stokes MP, Hui L, Silva JC, Overvoorde J, Giansanti P, Heck AJR, Kontaridis MI, den Hertog J and Bennett AM

Mol Cell Biol 34, 2874–2889

Download|2014

Mutant PTEN in cancer: worse than nothing

Leslie NR and den Hertog J

Cell 157, 527-529

Download|2014

Protein tyrosine phosphatases Epsilon and Alpha perform non-redundant roles in osteoclasts

Finkelshtein E, Lotinun S, Levy-Apter E, Arman E, den Hertog J, Baron R and Elson A

Mol Biol Cell, 25, 1808-1818

Download|2014

Noonan and LEOPARD syndrome Shp2 variants induce heart displacement defects in zebrafish

Bonetti M, Paardekooper Overman J, Tessadori F, Noël E, Bakkers J and den Hertog J

Development 141, 1961-1970

Download|2014

Distinct and overlapping functions of ptpn11 genes in zebrafish development

Bonetti M, Rodriguez-Martinez V, Paardekooper Overman J, Overvoorde J, van Eekelen M, Jopling C and den Hertog J

PLoS ONE, 9, e94884

Download|2014

Blood 123, 184-190

Download|2014

Integrating chemical and genetic silencing strategies to identify host kinase-phosphatase inhibitor networks that control bacterial infection

Albers HMHG, Kuijl C, Bakker J, Hendrickx L, Wekker S, Farhou N, Liu N, Blasco-Moreno B, Scanu T, den Hertog J, Celie P, Ovaa H and Neefjes J

ACS Chem Biol 9, 414-422

Download|2014

A versatile spectrophotometric protein tyrosine phosphatase assay based on 3-nitrophosphotyrosine containing substrates

van Ameijde J, Overvoorde J, Knapp S, den Hertog J, Ruijtenbeek R, Liskamp RM

Anal Biochem. 448C, 9-13

Download|2014

Zebrafish as a model to study PTPs during development

Paardekooper Overman J and den Hertog J

Methods 65, 247-253

Download|

A Nodal-independent and tissue-intrinsic mechanism controls heart-looping chirality

Noël ES, Verhoeven M, Lagendijk AK, Tessadori F, Smith K, Choorapoikayil S, den Hertog J, Bakkers J

Nat Commun 4, 2754

Download|2013

Loss of Pten promotes angiogenesis and enhanced vegfaa expression in zebrafish

Choorapoikayil S, Weijts B, Kers R, de Bruin A, den Hertog J

Dis Mod Mech, 6, 1159-1166

Download|2013

Deriving Cell Lines from Zebrafish Embryos and Tumors

Choorapoikayil S, Overvoorde J, den Hertog J

Zebrafish, 10, 316-325

Download|2013

Protein tyrosine phosphatases in health and disease

Hendriks WJ, Elson A, Harroch S, Pulido R, Stoker A, den Hertog J

FEBS J, 280, 708–730

Download|2013

Protein tyrosine phosphatase structure-function relationships in regulation and pathogenesis

Böhmer F, Szedlacsek S, Tabernero L, Ostman A, den Hertog J (

FEBS J., 280, 413–431

Download|2013

A variant in the carboxyl-terminus of connexin 40 alters GAP junctions and increases risk for tetralogy of Fallot

Guida V, Ferese R, Rocchetti M, Bonetti M, Sarkozy A, Cecchetti S, Gelmetti V, Lepri F, Copetti M, Lamorte G, Digilio MC, Marino B, Zaza A, den Hertog J, Dallapiccola B, De Luca A

Eur J Hum Gen 21, 69-75

Download|2013

Knocking Down Low Molecular Weight Protein Tyrosine Phosphatase (LMW-PTP) Reverts Chemoresistance through Inactivation of Src and Bcr-Abl Proteins

Ferreira PA, Ruela-de-Sousa RR, Queiroz KC, Souza AC, Milani R, Pilli RA, Peppelenbosch MP, den Hertog J, Ferreira CV

PLoS ONE 7, e44312

Download|2012

PLoS ONE 7, e35913

Download|2012

Dis Mod Mech, 5, 241-247

Download|2012

Pten Mediates Myc Oncogene Dependence in a Conditional Zebrafish Model of T-ALL

Gutierrez A, Grebliunaite R, Feng H, Kozakewich E, Zhu S, Guo F, Payne E, Mansour M, Dahlberg SE, Neuberg DS, den Hertog J, Prochownik EV, Testa JR, Harris M, Kanki JP, Look AT

J Exp Med, 208, 1595-1603

Download|2011

Noonan syndrome gain-of-function mutations in NRAS cause zebrafish gastrulation defects

Runtuwene V, van Eekelen M, Overvoorde J, Rehmann H, Yntema HG, Nillesen WM, van Haeringen A, van der Burgt I, Burgering B, den Hertog J

Dis Mod Mech 4, 393-399

Download|2011

PLoS ONE 5, e12573

Download|2010

In depth qualitative and quantitative profiling of tyrosine phosphorylation using a combination of phosphopeptide immuno-affinity purification and stable isotope dimethyl labeling

Boersema PJ, Foong LY, Ding VM, Lemeer S, van Breukelen B, Philp R, Boekhorst J, Snel B, den Hertog J, Choo AB, Heck AJ

Mol Cell Prot. 9, 84-99

Download|2010

Reciprocal regulation of RPTPalpha and Src

Vacaru, A.M. and den Hertog, J.

Rom. J. Biochem. 46, 187-197

Download|2009

Zebrafish and phosphatase function

den Hertog J, van Eekelen M

Handbook of Cell Signaling, Bradshaw R, Dennis E (eds) Chapter 101.

2009

Dev. Biol. 324, 122-130

Download|2008

Comparative phosphoproteomics of zebrafish Fyn/Yes morpholino knockdown embryos

Lemeer, S., Jopling, C., Gouw, J., Mohammed, S., Heck, A.J.R., Slijper, M. and den Hertog, J.

Mol. Cell. Prot., 7, 2176-2187

Download|2008

J. Cell Biol., 182, 1185-1200

Download|2008

Assessment of PTEN tumor suppressor activity in nonmammalian models: the year of the yeast

Cid, V.J., Rodrıguez-Escudero, I., Andres-Pons, A., Roma-Mateo, C., Gil, A., den Hertog, J., Molina, M. and Pulido, R.

Oncogene, 27, 5431-5442

Download|2008

FEBS J. 275, 2597–2604

Download|2008

Ferruginol suppresses survival signaling pathways in androgen-independent human prostate cancer cells

Bispo de Jesus, M, Zambuzzi, W.F., Ruela de Sousa, R.R., Areche, C. ... den Hertog, J., de Paula, E., Ferreira, C.V.

Biochimie, 90, 843-854

Download|2008

On-line automated zebrafish phosphoproteomics; From large-scale analysis down-to a single embryo

Lemeer, S., Pinkse, M.W.H., Mohammed, S., van Breukelen, B., den Hertog, J. Slijper, M. and Heck, A.J.R.

J. Prot. Res. 7, 1555-1564

2008

Protein tyrosine phosphatases: regulatory mechanisms

den Hertog, J. Östman, A and Böhmer, F.-D.

FEBS J. 275, 831–847

Download|2008

Zebrafish pten genes have overlapping and non-redundant functions in tumorigenesis and embryonic development

Faucherre, A., Taylor, G.S., Overvoorde, J., Dixon, J.E. and den Hertog, J.

Oncogene 27, 1079–1086

Download|2008

PLoS Genetics 3, e225

Download|2007

Endogenous phosphotyrosine signaling in zebrafish embryos

Lemeer, S.M., Ruijtenbeek, R., Pinkse, M.W.H., Jopling, C., Heck, A.J.R., den Hertog, J. and Slijper, M.

Mol. Cell. Prot. 6, 2088-2099

Download|2007

Protein-tyrosine kinase activity profiling in knock down zebrafish embryos

Lemeer, S., Jopling, C., Naji, F., Ruijtenbeek, R., Slijper, M., Heck, A.J.R. and den Hertog, J.

PLoS One 2(7): e581

Download|2007

Laminin alpha 4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and in endothelial cells

Knöll, R., Postel, R., Wang, J., Krätzner, R., Hennecke, G., Vacaru, A.M. ... Den Hertog, J., van Eeden, F.J.M., Peters, P.J., Hasenfuss, G., Chien, K.R.and Bakkers, J.

Circulation 116, 515-525

Download|2007

Mech. of Dev. 124, 129-136

Download|2007

Reversible oxidation of the membrane distal domain of receptor PTPalpha is mediated by a cyclic sulphenamide

Yang, J., Groen, A., Lemeer, S., Jans, A., Slijper, M., Roe, S.M., den Hertog, J. and Barford, D.

Biochemistry 46, 709-719

Download|2007

The novel gene asb11: a regulator of the size of the neural progenitor compartment

Diks, S.H., Bink, R.J., van de Water, S., Joore, J., van Rooijen, C., Verbeek, F.J., den Hertog, J., Peppelenbosch, M.P., Zivkovic, D.

J. Cell Biol. 174, 581-592

Download|2006

Type I collagen limits VEGFR-2 signaling by a SHP2 protein-tyrosine phosphatase – dependent mechanism

Mitola, S., Brenchio, B., Piccinini, M., Tertoolen, L., Zammataro, L., Breier, G., Rinaudo, M.T., den Hertog, J. and Bussolino, F.

Circ. Res. 98, 45-54

Download|2006

Integrin alpha4beta1 promotes FAK-independent cell motility via alpha4 cytoplasmic domain-specific activation of c-Src

Hsia, D.A., Lim, S.T., Bernard-Triflo, J.A., Mitra, S.K., Tanaka, S., den Hertog, J., Streblow, D.N., Ilic, D., Ginsberg, M.H. and Schlaepfer, D.D.

Mol. Cell. Biol. 25, 9700 – 9712

Download|2005

Cytotoxicity and recognition of receptor-like protein tyrosine phosphatases, RPTPalpha and RPTPbeta, by Helicobacter pylori m2VacA

De Guzman, B., Hisatsune, J., Nakayama, M., Yahiro, K., Wada, A., Yamasaki, E., Nishi, Y., Yamazaki, S., Azuma, T., Ito, Y., Ohtani, M., van der Wijk, T., den Hertog, J., Moss, J. and Hirayama, T.

Cell. Microbiol. 7, 1285-1293

Download|2005

Biosc. Rep., 25, 289-297

2005

Differential oxidation of protein-tyrosine phosphatases

Groen, A., Lemeer, S., van der Wijk, T., Overvoorde, J., Heck, A.J.R., Ostman, A., Barford, D., Slijper, M., and den Hertog, J.

J. Biol. Chem. 280, 10298-10304

Download|2005

Redox regulation of protein-tyrosine phosphatases

den Hertog, J., Groen, A. and van der Wijk, T.

Arch. Biochem. Biophys. 434, 11-15

Download|2005

RPTPalpha is essential for NCAM mediated p59fyn activation and neurite elongation

Bodrikov, V., Leshchyns’ka, I, Sytnyk, V., Overvoorde, J., den Hertog, J. and Schachner, M.

J. Cell Biol., 168, 127-139

Download|2005

Regulation of Receptor Protein-Tyrosine Phosphatase dimerization

van der Wijk, T., Blanchetot, C. and den Hertog, J

Methods 35, 73-79

Download|2005

Mol. Cell. Neurosci. 27, 404-416

Download|2004

J. Biol. Chem., 279, 44355-44361

Download|2004

Preferential oxidation of the second phosphatase domain of receptor-like PTP-revealed by an antibody against oxidized protein tyrosine phosphatases

Persson, C., Sjöblom, T., Groen, A., Kappert, K., Engström, U., Hellman, U., Heldin, C.H., den Hertog, J. and Östman, A.

Proc. Natl. Acad. Sci. USA, 101, 1886-1891

Download|2004

Top. Curr. Genet. (eds. J. Arino and D. Alexander) 17, 253-274

2004

EMBO Rep. 4, 1027-1032

Download|2003

Redox-regulated rotational coupling of Receptor Protein-Tyrosine Phosphatase α dimers

van der Wijk, T., Blanchetot, C., Overvoorde, J. and den Hertog, J.

J. Biol. Chem. 278,13968-13974

2003

Dimerization in vivo and inhibition of the non-receptor form of protein tyrosine phosphatase Epsilon

Toledano-Katchalski, H., Tiran, Z., Sines, T., Shani, G., Granot-Attas, S., den Hertog, J. and Elson, A.

Mol. Cell. Biol., 23, 5460-5471

Download|2003

Heparan sulfate 6-O-sulfotransferase is essential for muscle development in Zebrafish.

Bink, R.J., Habuchi, H., Lele, Z., Dolk, E., Joore, J., Rauch, G.J., Geisler, R., Wilson, S., den Hertog, J., Kimata, K. and Zivkovic, D.

J. Biol. Chem., 278, 31118-31127

Download|2003

Receptor Protein-Tyrosine Phosphatase dimerization

den Hertog, J., van der Wijk, T., Tertoolen, L.G.J. and Blanchetot, C.

Meth. Enzymol. (eds. S. Klumpp and J. Krieglstein) 366, 224-240

2003

Regulation of Receptor Protein Tyrosine Phosphatase alpha by oxidative stress

Blanchetot, C., Tertoolen, L.G., and den Hertog, J.

EMBO J., 21, 493-503

Download|2002

Dev.Dyn., 223, 292-297

Download|2002

J. Biol. Chem., 277, 47263-47269

Download|2002

J. Biol. Chem., 277, 48139-48145

Download|2002

A tumor-specific kinase activity regulates the viral death protein Apoptin

Rohn, J.L., Zhang, Y.H., Aalbers, R.I.J.M., Otto, N., den Hertog, J., Henriquez, N.V., van de Velde, C.J.H., Kuppen, P.J.K., Mumberg, D., Donner, P. and Noteborn, M.H.M.

J. Biol. Chem., 277, 50820-50827

Download|2002

Dimerization of Receptor Protein-Tyrosine Phosphatase alpha in living cells

Tertoolen, L.G., Blanchetot, C., Jiang, G., Overvoorde, J., Gadella, T.W.J., Hunter, T., and den Hertog, J.

BMC.Cell Biol. 2, 8

Download|2001

Expression of receptor protein-tyrosine phosphatase alpha, sigma and LAR during development of the zebrafish embryo

van der Sar, A., Betist, M., de Fockert, J., Overvoorde, J., Zivkovic, D., and den Hertog, J.

Mech.Dev. 109, 423-426.

Download|2001

Identification of p130cas as an in vivo substrate of receptor protein-tyrosine phosphatase alpha

Buist, A., Blanchetot, C., Tertoolen, L.G., and den Hertog, J.

J.Biol.Chem. 275, 20754-20761

Download|2000

Mol.Cell Biol. 20, 5917-5929

Download|2000

Biochemistry 38, 914-922

Download|1999

Growth factor signalling

de Laat, S.W., Boonstra, J., Defize, L.H., Kruijer, W., van der Saag, P.T., Tertoolen, L.G., van Zoelen, E.J., and den Hertog, J

Int.J.Dev.Biol. 43, 681-691

Download|1999

Mech.Dev. 85, 3-14

Download|1999

Receptor protein-tyrosine phosphatase signalling in development

den Hertog, J., Blanchetot, C., Buist, A., Overvoorde, J., van der Sar, A., and Tertoolen, L.G.

Int.J.Dev.Biol. 43, 723-733

Download|1999

Dimerization inhibits the activity of receptor-like protein-tyrosine phosphatase-alpha

Jiang, G., den Hertog, J., Su, J., Noel, J., Sap, J., and Hunter, T.

Nature 401, 606-610

Download|1999

J.Biol.Chem. 274, 27439-27447

Download|1999

Pleiotropic effects of zebrafish protein-tyrosine phosphatase-1B on early embryonic development

van der Sar, A.M., de Fockert, J., Betist, M., Zivkovic, D., and den Hertog, J.

Int.J.Dev.Biol. 43 , 785-794

Download|1999

Oncogene 18, 4435-4439

Download|1999

Biochem. Biophys. Res. Commun. 251, 6-10

Download|1998

Cell scattering of SK-N-MC neuroepithelioma cells in response to Ret and FGF receptor tyrosine kinase activation is correlated with sustained ERK2 activation

van Puijenbroek, A.A., van Weering, D.H., van den Brink, C.E., Bos, J.L., van der Saag, P.T., de Laat, S.W., and den Hertog, J

Oncogene 14, 1147-1157

Download|1997

Activin and basic fibroblast growth factor regulate neurogenesis of murine embryonal carcinoma cells

Ameerun, R.F., de Winter, J.P., van den Eijnden-van Raaij, A.J., den Hertog, J., de Laat, S.W., and Tertoolen, L.G.

Cell Growth Differ. 7, 1679-1688.

Download|1996

Nature 382, 555-559

Download|1996

Mech.Dev. 58, 89-101

1996

Why is phosphonodifluoromethyl phenylalanine a more potent inhibitory moiety than phosphonomethyl phenylalanine toward protein-tyrosine phosphatases?

Chen, L., Wu, L., Otaka, A., Smyth, M.S., Roller, P.P., Burke, T.R.J., den Hertog, J., and Zhang, Z.Y.

Biochem. Biophys. Res. Commun. 216, 976-984.

Download|1995

Stimulation of receptor protein-tyrosine phosphatase alpha activity and phosphorylation by phorbol ester

den Hertog, J., Sap, J., Pals, C.E., Schlessinger, J., and Kruijer, W.

Cell Growth Differ. 6, 303-307

Download|1995

Multiple and cooperative phosphorylation events regulate the CREM activator function

de Groot, R.P., den Hertog, J., Vandenheede, J.R., Goris, J., and Sassone-Corsi, P.

EMBO J. 12, 3903-3911

Download|1993

Receptor protein tyrosine phosphatase alpha activates pp60c-src and is involved in neuronal differentiation

den Hertog, J., Pals, C.E., Peppelenbosch, M.P., Tertoolen, L.G., de Laat, S.W., and Kruijer, W.

EMBO J. 12, 3789-3798

Download|1993

Epidermal growth factor induced signal transduction in A431 cells is influenced by altered gravity conditions

de Laat, S.W., de Groot, R.P., den Hertog, J., Rijken, P.J., Verkleij, A.J., Kruijer, W., and Boonstra, J.

Physiologist. 35, S19-S22

1992

Nucleic.Acids.Res. 20, 125-130

Download|1992

Biochem. Biophys. Res. Commun. 184, 1241-1249

Download|1992

Cell 69, 295-303

1992

Nuclear responses to protein kinase C signal transduction are sensitive to gravity changes

de Groot, R.P., Rijken, P.J., den Hertog, J., Boonstra, J., Verkleij, A.J., de Laat, S.W., and Kruijer, W.

Exp.Cell Res. 197, 87-90

Download|1991

Characterization of a tyrosine kinase signaling pathway in undifferentiated P19 embryonal carcinoma cells

den Hertog, J., Eman, R., Tertoolen, L.G., de Laat, S.W., and Kruijer, W.

Exp.Cell Res. 196, 226-232

1991

DNA Seq. 2, 111-119

1991

Microgravity decreases c-fos induction and serum response element activity

de Groot, R.P., Rijken, P.J., den Hertog, J., Boonstra, J., Verkleij, A.J., de Laat, S.W., and Kruijer, W.

J.Cell Sci. 97, 33-38

Download|1990

  • 1
  • 2
  • 3
  • 4
  • 5
  • 6
  • 7
  • 8
  • 9
  • 10
  • 11

Group leader

Jeroen den Hertog

Jeroen den Hertog is group leader and Deputy Director Research at the Hubrecht Institute and professor of Molecular Developmental Zoology at Leiden University. The Den Hertog group uses the zebrafish as a model system. They investigate the function of protein-tyrosine phosphatases (PTPs). PTP-signaling has an important role in many biological processes, including cell proliferation, migration and transformation. The Den Hertog group studies the regulation, substrates, ligands and effectors in PTP-signaling using biochemical, molecular and cell biological, and genetic approaches during zebrafish development and regeneration of the caudal fin.

Scientific training and positions

Current other activities


Group members

Jeroen den Hertog

Principal Investigator

John Overvoorde

Technician

Jelmer Hoeksma

Technician

Maja Šolman

Postdoc

Sasja Blokzijl-Franke

PhD Student

Petra Bakker

PhD Student

Maaike Allers

PhD Student

Xudong Ouyang

PhD Student

Reinier Keijnemans

Student

Job openings

Postdoc

Research group

den Hertog group

Start date

01/12/2018

Location

Utrecht Science Park

Period

Full time

Job openings

Postdoc

Research group

den Hertog group

Start date

01/12/2018

Location

Utrecht Science Park

Period

Full time