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Schulte-Merker group

Kok, F.O., Shin, M., Ni, C., Gupta, A., Grosse, A.S., van Impel, A., Kirchmaier, B.C., Peterson-Maduro, J., Kourkoulis, G., Male, I., DeSantis, D.F., Sheppard-Tindell, S., Ebarasi, L., Betsholtz, C., Schulte-Merker, S., Wolfe, S.A., and Lawson, N.D. (2015). Reverse Genetic Screening Reveals Poor Correlation between Morpholino-Induced and Mutant Phenotypes in Zebrafish. Dev Cell, in press.

Stainier, D.Y.R. and Schulte-Merker, S. (2014) Out with the old, in with the new: reassessing morpholino knock-downs in light of genome editing technology. Development, in press.

Apschner, A., Huitema, L.F.A., Ponsioen, B., Peterson-Maduro, J., and Schulte- Merker, S. (2014) Pathological mineralization in a zebrafish enpp1 mutant exhibits features of Generalized Arterial Calcification of Infancy (GACI) and Pseudoxanthoma Elasticum (PXE). Disease Models & Mechanisms, in press.

van Impel, A., Zhao, Z., Hermkens, D.M.A., Roukens, G., Fischer, J.C., Peterson-Maduro, J., Duckers, H., Ober, E.A., Ingham, P.W., and Schulte-Merker, S. (2014) Divergence of lymphatic cell specification pathways between zebrafish and mice. Development 141, 1228-1238.

Le Guen, L., Karpanen, T., Schulte, D., Harris, N.C., Koltowska, K., Roukens, G., Bower, N., van Impel, A., Stacker, S., Achen, M.G., Schulte-Merker, S.*, and Hogan, B.* (2014) Ccbe1 regulates Vegfc-mediated induction of Vegfr3 signaling during embryonic lymphangiogenesis. Development 141, 1239-1249.  [*joint senior authors]

van de Pavert, S., Ferreira, M., Domingues, R.G., Ribeiro, H., Molenaar, R., Moreira-Santos, L., Almeida, F.F., Ibiza, S., Barbosa, I., Labão-Almeida, I., Goverse, G., Godinho-Silva, C., Konijn, T., Schooneman, D., O’Toole, T., Mizee, M., Habani, Y., Haak, E., Santori, F.R., Littman, D.R., Schulte-Merker, S., Dzierzak, E., Simas, J.P., Mebius, R.E.,  and Veiga-Fernandes, H. (2014) Maternal retinoids control type 3 innate lymphoid cells and set the immune fitness of the progeny. Nature 508, 123-127.

Mackay, E., and Schulte-Merker, S. (2014) : A statistical approach to mutation detection in zebrafish with next-generation sequencing. J Appl Ichtyology, in press.

Geurtzen, K., Knopf, F., Wehner, D., Huitema, L.F.A., Schulte-Merker, S., and Weidinger, G. (2014) Mature osteoblasts dedifferentiate in response to traumatic bone injury in the zebrafish fin and skull. Development, 141, doi:10.1242/dev.105817.

Coxam, B., Sabine, A., Bower, N.I., Smith, K.A., Pichol-Thievend, C., Skoczylas, R., Astin, J.W., Frampton, E., Jaquet, M., Crosier, P.S., Parton, R.G., Harvey, N.L., Petrova, T.V., Schulte-Merker, S., Francois, M., and Hogan, B.M. (2014) Pkd1 regulates lymphatic vascular morphogenesis during development. Cell Reports, in press.

Kessels, M.Y., Huitema, L.F., Boeren, S., Kranenbarg, S., Schulte-Merker, S., van Leeuwen, J.L., and de Vries, S.C. (2014) Proteomics analysis of the zebrafish skeletal extracellular matrix. PLoS One 9(3):e90568. doi:10.1371

Mackay, E., Apschner, A., and Schulte-Merker, S. (2013) A Bone To Pick with Zebrafish. BoneKey, in press. doi:10.1038/bonekey.2013.179

van Impel, A. and Schulte-Merker, S. (2013) A Fisheye View on Lymphangiogenesis. In: Developmental Aspects of the Lymphatic Vascular System. Kiefer, F. and Schulte-Merker, S. (Eds.) Springer Press.

Postma, A.V., Alders, M., Sylva, M., Bilardo, C.M., Pajkrt, E., van Rijn, R.R., Schulte-Merker, S., Bulk, S., Stefanovic, S., Ilgun, A., Barnett, P., Mannens, M.M.A.M., Moorman, A.F.M., Oostra, R.J., and van Maarle, M.C. (2013). Mutations in the T gene cause a novel syndrome consisting of sacral agenesis, abnormal ossification of the vertebral bodies and a persistent notochordal canal. J Medical Genetics 51, 90-97.

Hägerling, R., Pollmann, C., Andreas, M., Schmidt, C., Nurmi, H., Adams, R.H., Alitalo. K.,  Andresen, V., Schulte-Merker, S., and Kiefer, F. (2013) A novel multi-step mechanism for inititial lymphangiogenesis in mouse embryos based on ultramicroscopy. EMBO J 32(5): 629-44.

Basten, S.G., Davis, E.E., Gillis, A.J., van Rooijen, E., Stoop, H., Babala, N., Logister, I., Heath, Z.G., Jonges, T.N., Katsanis, N., Voest, E.E., van Eeden, F.J., Medema, R.H., Ketting, R.F., Schulte-Merker, S., Looijenga, L.H., Giles, R.H. (2013) Mutations in LRRC50 Predipose Zebrafish and Humans to Seminomas. PLoS Genetics 9(4): e1003384.

Fritz, R.D., Letzelter, M., Reimann, A., Martin, K., Fusco, L., Ritsma, L., Ponsioen, B., Fluri, E., Schulte-Merker, S., van Rheenen, J., and Pertz, O., (2013). A versatile tool kit to produce sensitive FRET biosensors to visualize signaling in time and space. Science Signaling (6)285..

Helker, C.S.M., Schuermann, A., Karpanen, T., Zeuschner, D., Belting, H.-G., Affolter,M., Schulte-Merker, S., and Herzog, W. (2013). The Zebrafish Common Cardinal Veins Develop by a Novel Mechanism: 'Lumen Ensheathment'. Development 140 (13), 2776-86.

Gordon, K., Schulte, D., Brice, G., Simpson, M.A., Roukens, M.G., van Impel, A.W., Connell, F., Kalidas, K., Jefferey, S., Mortimer, P.S., Schulte-Merker, S., and Ostergaard, P. (2013) A mutation in VEGFC, a ligand for VEGFR3, is associated with autosomal-dominant Milroy-like primary lymphedema. Circulation Research 112(6), 956-60.

Huitema, L.F.A., Apschner, A., Logister, I., Spoorendonk, K.M., Bussmann, J., Hammond, C.L., and Schulte-Merker, S. (2012) Entpd5 is essential for skeletal mineralization and regulates phosphate homeostasis in zebrafish. PNAS 109 (52), 21372-7.

Mitchell, R.E., Huitema, L..F.A., Skinner, R..H.E., Brunt, L..H,  Severn, C.,Schulte-Merker, S. and Hammond C.L. (2012) New tools for studying osteoarthritis genetics in zebrafish. Osteoarthritis and Cartilage 21(2), 269-78.

Huitema, L.F.A., Renn, J., Logister, I., Waltz, S.E., Flik, G.,and Schulte-Merker, S. (2012) Macrophage stimulating protein is involved in skeletal mineralization and calcium homeostasis in zebrafish. FASEB Journal 26(10):4092-101.

Bart G.M.W. Weijts, Bakker, W.J., Cornelissen, P.W.A., Liang, K., Schaftenaar, F.H., Westendorp, B., de Wolf, C.A.C.M.T., Paciejewska, M., Scheele, C.L.G.J., Kent, L., Leone, G., Schulte-Merker, S., de Bruin, A. (2012) E2F7 and E2F8 promote angiogenesis through transcriptional activation of VEGFA in cooperation with HIF1. EMBO Journal 31(19): 3871-84..

Tempel, D., de Boer, M., van Deel, E.D., Haasdijk, R.A., Duncker, D.J., Cheng, C., Schulte-Merker, S., and Duckers, H.J. (2012) Apelin enhances cardiac neovascularization after myocardial infarction by recruiting Aplnr+ circulating cells. Circulation Research 111(5), 585-98.

Wilkinson, R.N., Koudijs, M., Ingham, P.W., Patient, R.K., Schulte-Merker, S., and van Eeden, F.J.M. (2012) Hedgehog signalling via a calcitonin receptor-like receptor can induce arterial differentiation independently of VEGF signalling in zebrafish. Blood 120 (2), 477-88.

Fiaz, A.W., Leon-Kloosterziel, K.M., Gort, G., Schulte-Merker, S., van Leeuwen, J.L., and Kranenbarg, S. (2012) Swim-training changes the spatio-temporal dynamics of skeletogenesis in zebrafish larvae (Danio rerio). PLoS One 7 (4): e34072.

Cheng, C., Haasdijk, R., Tempel, D., van de Kamp, E.H.M., Herpers, R., Bos, F., den Dekker, W.K., Blonden, L.A.J., de Jong, R., Bürgisser, P.E., Chifri, I., Biessen, E.A.L., Dimmeler, S., Schulte-Merker, S., and Duckers, H.J. (2012) Endothelial cell specific FGD5 involvement in vascular pruning defines neovessel fate in mice. Circulation 125(25), 3142-58.

Ostergaard, P., Simpson, M.A., Mendola, A., Vasudevan, P., Connell, F.C., van Impel, A., Moore, A.T., Loeys, B.L., Ghalamkarpour, A., Onoufriadis, A., Martinez-Corral, I., Devery, S., Leroy, J.G., van Laer, L., Singer, A., Bialer, M.G., McEntagart, M., Quarrell, O., Brice, G., Trembath, R.C., Schulte-Merker, S., Makinen, T., Vikkula, M., Mortimer, P.S., Mansour, S., and Jeffery, S. (2012). Mutations in KIF11 cause autosomal dominant microcephaly variably associated with congenital lymphedema and chorioretinopathy. American J of Human Genetics 90 (2): 356-62.

Schulte‐Merker, S., Sabine, A., and Petrova, T. (2011).  Mechanisms of lymphatic vascular morphogenesis in development, physiology and human disease.  Journal of Cell Biology 193, 607-618.

Bos, F.L., Caunt, M., Peterson-Maduro, J., Planas-Paz, L., Kowalski, J., Karpanen, T., van Impel, A., Tong, R., Ernst, J.A., Korving, J., van Es., J.H., Lammert, E., Duckers, H.J., and Schulte-Merker, S. (2011) CCBE1 Is Essential for Mammalian Lymphatic Vascular Development and Enhances the Lymphangiogneic Effect of Vascular Endothelial Growth Factor-C In Vivo. Circulation Research109(5): 486-91.

Tao, S., Witte, M., Bryson-Richardson, R.J., Currie, P.D., Hogan, B.M., and Schulte-Merker, S. (2011). Zebrafish prox1b mutants develp a lymphatic vasculature, and prox1b does not specifically mark lymphatic endothelial cells. PLoS One 6(12):e28934.

Vanoevelen, J., Janssens, A., Huitema, L.F.A., Hammonds, C.L., Metz, J.R., Flik, G., Voets, T., and Schulte-Merker, S. (2011). Trpv5/6 is vital for epithelial calcium uptake and bone formation. FASEB Journal 25(9): 3197-207.

Bussmann, J. and Schulte-Merker, S. (2011) Rapid BAC selection for tol2-mediated transgenesis in zebrafish. Development 138, 4327-4332..

Fiedler, J., Jazbutyte, V., Kirchmaier, B.C., Kumar Gupta, S., Lorenzen, J., Hartmann, D., Galuppo, P., Kneitz, S., Pena, J.T.G., Sohn-Lee, C., Loyer, X., Soutschek, J., Brand, T., Tuschl, T., Heineke, J., Martin, U., Schulte-Merker, S., Ertl, G., Engelhardt, S., Bauersachs, J., and Thum, T. (2011). MicroRNA-24 regulates vascularity after myocardial infarction. Circulation 124(6): 720-30.

Cheng, C., Tempel, D., den Dekker, W., Haasdijk, R., Chrifi, I., Bos, F., Wagtmans, K., van de Kamp, E.H., Blonden, L., Biessen, E.A.L., Moll, F., Pasterkamp, G., Serruys, P.W., Schulte-Merker, S., and Duckers, H.J. (2011) Ets2 determines the inflammatory state of endothelial cells in advanced atherosclerotic lesions. Circulation Research 109(4): 382-95.

Knopf, F., Hammond, C., Chekuru, A., Kurth, T., Hans, S., Weber, C.W., Mahatma, G., Fisher, S., Brand, M., Schulte-Merker, S., and Weidinger, G. (2011). Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin. Developmental Cell 20, 713-724.

Krueger, J., Liu, D., Katja , K., Zimmer, A., Shi,Y., Klein, C., Siekmann, A., Schulte-Merker, S., Cudmore, M., Ahmed, A., and le Noble, A.F. (2011). Flt1 acts as a negative regulator of tip cell formation and branching morphogenesis in the zebrafish embryo. Development 138, 2111-2120.

Kärpänen, T and Schulte-Merker, S. (2011). Zebrafish provides a novel model for lymphatic vascular research. Methods in Cell Biolgy, Elsevier (105: 223-238).

Apschner, A., Schulte-Merker, S. and Witten, P.E. (2011). Not all bones are created equal - using zebrafish and other teleost species in osteogenesis research. Methods in Cell Biology, Elsevier (105:239-55).

van Rooijen, E., Santhakumar, K., Logister, I., Voest, E., Schulte-Merker, S., Giles, R., and Schulte-Merker, S. (2011). A Zebrafish Model for VHL and Hypoxia Signaling. Methods in Cell Biology, Elsevier (105: 163-90).

Roukens, M.G., Alloul-Ramdhani, M., Baan, B., Kobayashi, K., Peterson-Maduro, J., van Dam, H., Schulte-Merker, S., and David A. Baker. (2010). Control of endothelial sprouting by a Tel-CtBP complex. Nature Cell Biology 12, 933-43.

Jakobsson, L., Franco, C.A., Bentley, K., Collins, R.T., Ponsioen, B., Aspalter, I.M., Rosewell, I.,Marta Busse, Thurston, G., Medvinsky, A., Schulte-Merker, S., and Holger Gerhardt. (2010). Endothelial cells dynamically compete for the tip cell position during angiogenic sprouting. Nature Cell Biology 12(10), 943-53.

Gjini, E., Hekking, L., Kuchler, A., Saharinen, P., Wienholds, E., Post, J.-A., Alitalo, K., and Schulte-Merker, S. (2010). Zebrafish Tie-2 shares redundant roles with Tie-1 in heart development and regulates vessel integrity. Disease Models & Mechanisms 4, 57-66.

Bussmann, J., Bos, F., Urasaki, A., Kawakami, K., Duckers, H.J., and Schulte-Merker, S. (2010). Arteries provide essential guidance cues for lymphatic endothelial cells in the zebrafish trunk. Development 137, 253-57.

Hermans, K., Claes, F., Wouter Vandevelde; Wei Zheng; Ilse Geudens; Fabrizio Orsenigo; Frederik De Smet; Evisa Gjini; Kristof Anthonis; Bin Ren; Dontscho Kerjaschki; Monica Autiero; Annelii Ny; Michael Simons; Mieke Dewerchin; Stefan Schulte-Merker; Elisabetta Dejana; Kari Alitalo, Carmeliet, P. (2010) Role of synectin in lymphatic development in zebrafish and frogs. Blood 116(17), 3356-66.

Meta-analysis of genome-wide association studies confirms a susceptibility for knee-osteoarthritis on chromosome 7q22. (2010) Evangelou, E., Valdes, A.M., Kerkhof, H.J.M., et al. Annals of the Rheumatic Diseases doi:10.1136/ard.2010.132787.

Geudens, I., Herpers, R., Hermans, K., Segura, I., Ruiz de Almodovar, C., Bussmann, J., De Smet, F., Vandevelde, W., Hogan, B.M., Siekmann, A., Claes, F., Moore, J.C., Pistocchi, A.S., Loges, S., Mazzone,M., Mariggi, G., Bruye`re, F., Cotelli, F., Kerjaschki, D., Noe¨l, A., Foidart, J.-M., Gerhardt, H., Ny, A., Langenberg, T., Lawson, N.D., Duckers, H.J., Schulte-Merker, S., Carmeliet, P., Dewerchin, M. (2010). Role of Dll4/Notch in the Formation and Wiring of the Lymphatic Network in Zebrafish. Arterioscler Thromb Vasc Biol. 30(9), 1695-1702.

Pedrioli, D.M.L., Karpanen, T., Dabouras, V., Jurisic, G., van de Hoek, G., Shin, J.W., Marino, D., Kälin, R.E., Leidel, S., Cinelli, P., Schulte-Merker, S., Brändli, A.W., and Michael Detmar. (2010) miR-31 functions as a negative regulator of lymphatic vascular lineage-specific differentiation in vitro and vascular development in vivo. Molecular and Cellular Biology, doi:10.1128/MCB.00185-10 .

Tournoij, E., Weber, G.J., Akkerman, J.N., de Groot, P.G., Zon, L.I., Moll, F., and Schulte-Merker, S. (2010) Mlck1a is expressed in zebrafish thrombocytes and an essential component for thrombus formation. Journal of Thrombosis and Haemostasis 8, 588-595.

van Rooijen, E., Voest, E.E., Logister, I., Bussmann, J., Korving, J., van Eeden, F.J., Giles, R.H., and Schulte-Merker, S. (2010)  von Hippel-Lindau tumor suppressor mutants faithfully model pathological hypoxia-driven angiogenesis and vascular retinopathies in zebrafish. Disease Models & Mechanisms 3, 343-353.

Spoorendonk, K.M., Hammond, C.L., Huitema, L.F.A., Vanoevelen, J., and Schulte-Merker, S. (2010). Zebrafish as a unique model system in bone research: the power of genetics and in vivo imaging. Journal of Applied Ichthyology 26, 219-224.

Alders, M., Hogan, B.M., Gjini, E., Salehi, F., Al-Gazali, L., Hennekam, E.A., Holmberg, E.E., Mannens, M.M.A.M., Mulder, M.F., Offerhaus, G.J.A., Prescott, T.E., Schroor, E.J., Verheij, J.B.G.M., Witte, M., Zwijnenburg, P.J., Vikkula, M., Schulte-Merker, S. & Hennekam, R.C. (2009) Mutations in CCBE1 cause generalized lymph vessel dysplasia in humans. Nature Genetics, published online: 22 November 2009 | doi:10.1038/ng.484

Hammond, L.C., and Schulte-Merker, S. (2009) Two populations of endochondral osteoblasts with differential sensitivity to Hedgehog signaling. Development 136, 3991-4000.

Hogan, B.M., Herpers, R., Witte, M., Heloterä, H., Alitalo, K., Duckers, H.J., and Schulte-Merker, S. (2009) Vegfc/Flt4 signalling is suppressed by Dll4 in developing zebrafish intersegmental arteries. Development 136, 4001-4009.

Hogan, B.M., Bos, F., Bussmann, J., Witte, M., Chi, N., Duckers, H.,and Schulte-Merker, S. (2009). Ccbe1 is required for embryonic lymphangiogenesis and venous sprouting. Nature Genetics 41 , 396-398.

van Rooijen, E., Voest, E.E., Logister, I., Korving, J., Schwerte, T., Schulte-Merker, S., Giles, R.H., and van Eeden, F.J. (2009). Zebrafish mutants in the von Hippel-Lindau (VHL) tumor suppressor display a hypoxic response and recapitulate key aspects of Chuvash polycythemia. Blood 113, 6449-6460.

Voss, K., Stahl, S., Hogan, B.M., Reinders, J., Schleider, E., Schulte-Merker, S., and Felbor, U. (2009). Functional analyses of human and zebrafish 18 amino acid in frame deletion pave the way for domain mapping of the cerebral cavernous malformation 3 (CCM3) protein. Human Mutation 30(6), 1003-1011.

Brittijn SA, Duivesteijn SJ, Belmamoune M, Bertens LF, Bitter W, de Bruijn JD, Champagne DL, Cuppen E, Flik G, Vandenbroucke-Grauls CM, Janssen RA, de Jong IM, de Kloet ER, Kros A, Meijer AH, Metz JR, van der Sar AM, Schaaf MJ, Schulte-Merker S, Spaink HP, Tak PP, Verbeek FJ, Vervoordeldonk MJ, Vonk FJ, Witte F, Yuan H, Richardson MK. (2009) Zebrafish development and regeneration: new tools for biomedical research. Int J Dev Biology 53 (5-6), 835-50.

Hogan, B.M., and Schulte-Merker, S. (2009). New animal models of lymphangiogenesis. In: Lymphangiogenesis in cancer metastasis. Springer press.

Spoorendonk, K., Peterson-Maduro, J., Renn, J., Trowe, T., Kranenbarg, S., Winkler, C., and Schulte-Merker, S. (2008). Retinoic Acid and Cyp26b1 are Critical Regulators of Osteogenesis in the Axial Skeleton. Development 135, 3765-3774.

Hogan, B.M., Bussmann, J., Wollburg, H. and Schulte-Merker, S. (2008). ccm1 cell autonomously regulates endothelial cellular morphogenesis and vascular tubulogenesis in zebrafish. Human Molecular Genetics 17, 2424-2432.

Bussmann, J., Lawson, N., Zon, L., Schulte-Merker, S., and Zebrafish Nomenclature Committee. (2008). Zebrafish VEGF Receptors: A Guideline to Nomenclature. PLoS Genetics 4(5):e1000064.

Herpers, R., van de Kamp, E., Duckers, H.J., and Schulte-Merker, S. (2008). Redundant roles for sox7 and sox18 in arteriovenous specification in zebrafish. Circulation Research 102, 12-15.

van Rooijen, E., Giles, R.H., Voest, E.E., van Rooijen, C., Schulte- Merker, S. and van Eeden, F.J. (2008). LRRC50, a conserved ciliary protein implicated in polycystic kidney disease. J. American Society of Nephrology 19: 1128-1138.

Smith, K.A., Chocron, S., von der Hardt, S., de Pater, E., Soufan, A., Bussmann, J., Schulte-Merker, S., Hammerschmidt, M., and Bakkers, J. (2008). Rotation and asymetric development of the zebrafish heart requires directed migration of cardiac progenitor cells. Developmental Cell 14, 287-297.

Bussmann, J., Bakkers, J., and Schulte-Merker, S. (2007). Early endocardial morphogenesis requires Scl/Tal1. PLoS Genetics, 3(8): e140.

Küchler, A.M., Gjini, E., Peterson-Maduro, J., Cancilla, B., Wolburg, H., Schulte-Merker, S. (2006). Development of the zebrafish lymphatic system requires Vegfc signaling. Current Biology 16, 1244-1248.

Stéphane Berghmans, Ryan D. Murphey, Erno Wienholds, Donna Neuberg, Jeffrey L. Kutok, Christopher D.M. Fletcher, John P. Morris, Ting-Xi Liu, Stefan Schulte-Merker, John P. Kanki, Ronald Plasterk , Leonard I. Zon, A. Thomas Look. (2005). Malignant peripheral nerve sheath tumors in homozygous p53 mutant zebrafish. PNAS 102, 407-412.

Schulte-Merker, S. (2003). Genetics and Genomics in the Zebrafish - from Gene to Function and Back. Model Organisms in Drug Discovery, p. 185-201.

Martyn, U. and Schulte-Merker, S. (2003). The ventralized ogon mutant phenotype is caused by a defect in the zebrafish sizzled gene. Developmental Biology 260, 58-67.

Habeck, H., Odenthal, J., Walderich, B., Maischein, H.-M., Tübingen 2000 screen consortium, and Schulte-Merker, S. (2002). Analysis of a zebrafish VEGF receptor mutant reveals specific disruption of angiogenesis. Current Biology 12, 1405-1412.

Schulte-Merker, S. (2002). Looking at embryos. Zebrafish - A Practical Approach, p. 39-58.

Wienholds, E., Schulte-Merker, S., Walderich, B., and Plasterk, R. (2002). Target-selected inactivation of the zebrafish rag1 gene. Science 297, 99-102.

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