Delivery of newly synthesized proteins to the plasma membrane and the extracellular medium takes place in the membrane organelle of the secretory pathway. The molecular machinery behind the functional organization of this pathway is tightly controlled and is regulated by signaling. In this context, we study the role of ERK7 in Drosophila S2 cells. In addition to this classical secretion, a number of transmembrane proteins are unconventionally deposited to the plasma membrane, a pathway whose biogenesis is triggered by mechanical stress at specific stages of development. We study this unconventional secretion pathway in Drosophila as well as mammalian cells using genetics and microscopy.
About the research
Selected recent key publications
Herpers, B., Xanthakis, D. Rabouille, C. (2010) ISH-IEM: A sensitive method to detect endogenous mRNAs at the ultrastructural level. Nat. Protoc. 5:678-687.
Nickel, W., Rabouille, C. (2009) Mechanisms of regulated unconventional protein secretion. Nat. Rev. CellMol. Biol. 10:148-155.
Schotman, H., Karhinen, L., Rabouille, C. (2008) dGRASP mediated non-conventional integrin secretion is required for epithelial integrity. Dev. Cell 14:171-182.
Delanoue, R., Herpers, B., Soetaert, J., Davis, I., Rabouille, C. (2007) Drosophila Squid/hnRNP Helps Dynein Switch from a gurken mRNA Transport Motor to an Ultrastructural Static Anchor in Sponge Bodies. Dev. Cell13: 523-538.
Kondylis, V., van Nispen tot Pannerden, H.E., Herpers, B., Friggi-Grelin, F., Rabouille, C. (2007). The Golgi comprises a paired stack that is separated at G2 by modulation of the actin cytoskeleton through Abi and Scar/WAVE. Dev. Cell12: 901-915.