Meijlink Group

Genetics of mammalian development

The Meijlink group investigates fundamental processes that direct mammalian embryonic development. To this end we take advantage of the mouse as an in vivo model system, utilising two different genetic approaches.
We have initiated a forward screen for developmental mutations. This involves backcrossing of mutagen-treated mice, followed by identification of mutant lines on the basis of observed malformed embryos. This approach has allowed identification of a number of new mutants that we are currently analysing and mapping (collaboration with the Cuppen group in the Institute). In these mutants we see neural tube closure defects, cardiac malformations and early gastrulation defects.
Using gene targeting technology we have inactivated members of the ‘aristaless' family of paired-type homeobox genes, and characterised the resulting skeletal and cardiac defects.

About the research

Key publications
Blaschke, R.J., Hahurij, N.D., Kuijper, S., Wisse, L.J., Deissler, K., Hubert, A., Just, S., Anastassiadis, K., Spitzer, J., Schöler, H., Feitsma, H., Rottbauer, W., Blum, M., Meijlink, F., Rappold, G., Gittenberger-de Groot, A.C. (2007) Targeted mutation reveals essential functions of the homeodomain transcription factor Shox2 in sinoatrial and pacemaking development. Circulation 115: 1830-1838.

Kuijper, S., Feitsma, H., Sheth, R., Korving, J., Reijnen, M., Meijlink, F. (2005) Function and regulation of Alx4 in limb development: complex genetic interactions with Gli3 and Shh. Dev. Biol. 285: 533-544.

Kuijper, S., Beverdam, A., Kroon, C., Brouwer, A., Candille, S., Barsh, G., Meijlink, F. (2005) Genetics of shoulder girdle formation: roles of Tbx15 and aristaless-like genes. Development 132: 1601-1610.
Oosterveen, T., van Vliet, P., Deschamps, J., Meijlink, F. (2003) The direct context of a Hox retinoic acid response element is crucial for its activity. J. Biol. Chem. 278: 24103-24107.

te Welscher, P., Zuniga, A., Kuijper, S., Drenth, T., Goedemans, H.J., Meijlink, F., Zeller, R. (2002). Progression of vertebrate limb development through SHH-mediated counteraction of GLI3. Science 298: 827-830.

Publication list