A major unresolved challenge in biology is to decipher how cells can exist in the diverse cellular states that make up a functional organism while carrying the same genomic information.
Cell identity is determined by developmental programs that use select transcription factors that impose an epigenetic signature upon the genome thus enforcing select gene expression programs. These signatures can often be found deregulated in developmental abnormalities and later onset complex disease.
Our goal is to understand the complexity of the epigenetic landscape and its role during development, and disease progression through large scale ChIP-sequencing analysis. We combine this with in vitro stem cell based assays and model organisms to use this large scale information in order to control cell state changes.
About the research
Creyghton, M.P., Cheng, A., Welstead, G.G., Kooistra, T., Carey, B.W., Steine, E.J., Hanna, J., Lodato, M.A., Frampton ,G.M., Sharp, P.A., Boyer, L.A., Young, R.A., Jaenisch, R. (2010) Histone H3K27ac seperates active from poised enhancers and predicts developmental state. Proc. Natl. Acad. Sci. 107: 21931-21936.
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