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Clevers group

Lgr5 stem cells, Wnt signaling & cancer

Originally focused on T lymphocyte transcription factors, we cloned Tcf1 in 1991. With the discovery that Tcf factors are the final effectors of Wnt signaling, we changed our interests to the biology of Wnt signaling in intestinal self-renewal and cancer.  We identified a series of adult tissue stem cells with the novel Lgr5 marker and technologies for long-term culture of these stem cells as epithelial organoids, currently our major focus of research.  For a more detailed overview, click here.

About the research

Fast Facts: Gut feeling and science from Fast Facts on Vimeo.


Key publications
(5 recent)
Schwank G, Koo BK, Sasselli V, Dekkers JF, Heo I, Demircan T, Sasaki N, Boymans S, Cuppen E, van der Ent CK, Nieuwenhuis EE, Beekman JM and Clevers H. Functional repair of CFTR by CRISPR/Cas9 in intestinal stem cell organoids of cystic fibrosis patients.
Cell Stem Cell 13: 653-658 (2013)

Huch. M., Dorell, C., Boj, S.F., van Es, J.H., van de Wetering, M., Li, V.S.W., Hamer, K., Sasaki, N., Finegold, M.J., Haft, A., Grompe, M., Clevers, H. In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.
Nature 494: 247-250 (2013)

de Lau, W., Barker, N., .. and Clevers, H. Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling.
Nature 476: 293-297 (2011)

Sato, T., Vries, R., Snippert, H., van de Wetering, M., Barker, N., Stange, D., van Es, J., Abo, A., Kujala, P., Peters, P., Clevers, H. Single lgr5 gut stem cells build crypt-villus structures in vitro without a stromal niche 459(7244):262-5
Nature 459 : 262-265 (2009)

Barker, N., van Es, J.H., Kuipers, J., Kujala, P., van den Born, M., Cozijnsen, M., Korving, J., Begthel, H., Peters, P.C., Clevers, H. Identification of stem cells in small intestine and colon by a marker gene LGR5. Nature 449: 1003-1007 (2007)

Publication list