Jacco van Rheenen

Jacco van Rheenen was originally trained in a variety of imaging techniques during his PhD with Dr. Kees Jalink at the Netherlands Cancer Institute. He was among the first to optimize imaging and develop software to quantitatively measure FRET on confocal microscopes. During his PhD in the lab of Dr. Jalink and as postdoc in the lab of Dr. Sonnenberg (Netherlands Cancer Institute) he used several microscopy techniques to study lipid signaling in tumor cells. In order to broader his scales, he obtained a KWF fellowship to do a postdoc in the United States in the lab of Dr. John Condeelis. There he extended his imaging experience by imaging mammary tumors intravitally including two-photon microscopy and became an expert in the field of intravital FRET imaging. In 2008 he was appointed as group leader at the Hubrecht Institute, where he utilizes his imaging techniques to visualize processes that are required for the metastasis of mammary tumor cells in living animals. In 2009, he was awarded a VIDI grant and a research grant from the Dutch Cancer Society. In 2012, he was awarded a research grant from the Association for International Cancer Research (who have now rebranded to Worldwide Cancer Research), and in 2013 a research grant from Netherlands Organisation for Scientific Research. In 2013, he received the Stem Cells Young Investigator Award (see video below). In July 2014 he was appointed professor in Intravital Microscopy at the University Medical Center Utrecht. In 2015, he was awarded an ERC consolidator grant.

j.vanrheenen(at)hubrecht.eu

Team members van Rheenen

Ronny Schafer

Technician

Ronny SchÑfer

Carrie Maynard

PhD Student

Carrie Maynard

Pim Toonen

Technician

Pim Toonen

Colinda Scheele

PhD Student

Colinda Scheele

Anko de Graaff

Technician

Anko de Graaff

Saskia Ellenbroek

Postdoc

Saskia Ellenbroek

Arianna Fumagalli

PhD Student

Ariana Fumagalli

Maria Alieva Krasheninnikova

Postdoc

Maria Alieva

Danielle Seinstra

PhD Student

Danielle Seinstra

Sander Steenbeek

PhD Student

Sander Steenbeek

Jacco van Rheenen

vanrheenen

Saskia Suijkerbuijk

Postdoc

Saskia Suijkerbuijk

Anoek Zomer

PhD Student

Anoek Zomer

Lotte Bruens

PhD Student

Lotte Bruens

Evelyne Beerling

Technician

Evelyne Beerling

Show more

Show more

Research

Van Rheenen: Cancer biophysics

Van Rheenen: Cancer biophysics

Complication of metastasis, the process in which cells detach from the primary tumor to form new distant tumor sites, is the primary reason why patients die from cancer. Most of our knowledge of tissue homeostasis, tumor initiation, progression and metastasis, and chemotherapy resistance is derived from techniques that draw a static picture of these highly dynamic processes. To study the dynamic aspects of these processes, the van Rheenen group develops state-of-the-art imaging techniques to visualize and study individual cells in real-time in living animals, referred to as intravital microscopy. By these experiments we hope to indetify new drug targets...

Read research

Key publications

Zomer A, Maynard C, Verweij FJ, Kamermans A, Schäfer R, Beerling E, Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SIJ, Wurdinger T, Pegtel DM, van Rheenen J, (2015), In Vivo Imaging Reveals Extracellular Vesicle-Mediated Phenocopying of Metastatic Behavior. Cell, 161(5):1046–1057 Zomer_et_al_extended pdf

 

Ellenbroek SI, van Rheenen J. (2014) Imaging hallmarks of cancer in living mice. Nature Reviews Cancer, 14(6): 406-18

 

Ritsma L*, Ellenbroek SI*, Zomer A, Snippert HJ, de Sauvage FJ, Simons BD, Clevers H, van Rheenen J, (2014) Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging. Nature, 507(7492): 362-5

 

Ritsma L, Vrisekoop N, van Rheenen (2013) In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique. Nat Commun., 4:2366

 

Ritsma, L.*, Steller, E.J.A.*, Beerling, E., Loomans, C.J.M., Zomer, A., Gerlach, C., Vrisekoop, N., Seinstra, D., van Gurp, L., Schäfer, R., Raats, D.A., de Graaff, A. Schumacher, T.N., de Koning, E.J.P. Borel Rinkes, I.H., Kranenburg, O. van Rheenen, J. (2012) Intravital Microscopy Through an Abdominal Imaging Window Reveals a Pre-Micrometastasis Stage During Liver Metastasis. Sci Transl Med 4, 158ra145.

 

Jalink K, van Rheenen, J (2010) Nano-imaging of membrane topography affects interpretations in cell biology. Nat. Meth. 7:486.

 

Snippert HJ, van der Vlier LG, Sato T, van Es JH, van der Born M, Kroen-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. (2010) Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells, Cell, 143(1): 134-144.

All publications

In Vivo imaging reveals extracellular vesicle-mediated phenocopying of metastatic behavior
2015

Anoek Zomer, Carrie Maynard, Frederik Johannes Verweij, Alwin Kamermans, Ronny Schäfer, Evelyne Beerling, Raymond Michel Schiffelers, Elzo de Wit, Jordi Berenguer, Saskia Inge Johanna Ellenbroek, Thomas Wurdinger, Dirk Michiel Pegtel, Jacco van Rheenen

PTP1B-dependent regulation of receptor tyrosine kinase signaling by the actin-binding protein Mena
2015

Shannon K Hughes, Madeleine J Oudin, Jenny Tadros, Jason Neil, Amanda Del Rosario, Brian A Joughin, Laila Ritsma, Jeff Wyckoff, Eliza Vasile, Robert Eddy, Ulrike Philippar, Alisha Lussiez, John S Condeelis, Jacco van Rheenen, Forest White, Douglas A Lauffenburger, Frank B Gertler

Imaging hallmarks of cancer in living mice
2014

Saskia I J Ellenbroek, Jacco van Rheenen

Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging
2014

Laila Ritsma, Saskia I J Ellenbroek, Anoek Zomer, Hugo J Snippert, Frederic J de Sauvage, Benjamin D Simons, Hans Clevers, Jacco van Rheenen

The mechanisms and physiological relevance of glycocalyx degradation in hepatic ischemia/reperfusion injury
2014

Rowan F van Golen, Megan J Reiniers, Nienke Vrisekoop, Coert J Zuurbier, Pim B Olthof, Jacco van Rheenen, Thomas M van Gulik, Barry J Parsons, Michal Heger

Surgical implantation of an abdominal imaging window for intravital microscopy
2013

L. Ritsma, E.J. Steller, S.I. Ellenbroek, O. Kranenburg, I.H. Rinkes, J. van Rheenen

A versatile toolkit to produce sensitive FRET biosensors to visualize signaling in time and space
2013

R.D. Fritz, M. Letzelter, A. Reimann, K. Martin, L. Fusco, L. Ritsma, B. Ponsioen, E. Fluri, S. Schulte-Merker, J. van Rheenen, O. Pertz

Intravital imaging of cancer stem cell plasticity in mammary tumors
2013

A. Zomer, S.I. Ellenbroek, L. Ritsma, E. Beerling, N. Vrisekoop, J. van Rheenen

In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique
2013

L. Ritsma, N. Vrisekoop, J. van Rheenen

Intravital FRET imaging of tumor cell viability and mitosis during chemotherapy
2013

A. Janssen, E. Beerling, R. Medema, J. van Rheenen

Tissue-resident memory CD8+ T cells continuously patrol skin epithelia to quickly recognize local antigen
2012

S. Ariotti, J.B. Beltman, G. Chodaczek, M.E. Hoekstra, A.E. van Beek, R. Gomez-Eerland, L. Ritsma, J. van Rheenen, A.F. Maree, T. Zal, R.J. de Boer, J.B. Haanen, T.N. Schumacher

Intravital microscopy through an abdominal imaging window reveals a pre-micrometastasis stage during liver metastasis
2012

L. Ritsma, E.J. Steller, E. Beerling, C.J. Loomans, A. Zomer, C. Gerlach, N. Vrisekoop, D. Seinstra, L. van Gurp, R. Schafer, D.A. Raats, A. de Graaff, T.N. Schumacher, E. de Koning, I.H. Rinkes, O. Kranenburg, J. van Rheenen

Intravital imaging of cell signaling in mice
2012

L. Ritsma, B. Ponsioen, J. van Rheenen

If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer
2012

L. Bonapace, J. Wyckoff, T. Oertner, J. van Rheenen, T. Junt, M. Bentires-Alj

Real-time intravital imaging of cancer models
2011

A.W.M. Zomer, E. Beerling, E.J. Vlug, J. van Rheenen

The death receptor CD95 activates the cofilin pathway to stimulate tumour cell invasion
2011

E.J. Steller, L. Ritsma, D.A. Raats, F.J. Hoogwater, B.L. Emmink, K.M. Govaert, J. Laoukili, I.H. Rinkes, J. van Rheenen, O. Kranenburg

Intravital microscopy: new insights into metastasis of tumors
2011

E. Beerling, L. Ritsma, N. Vrisekoop, P.W. Derksen, J. van Rheenen

Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells
2010

H.J.G. Snippert, L.G. van der Flier, T. Sato, J.H. van Es, M.M.W. van den Born, C. Kroon-Veenboer, N. Barker, A.M. Klein, J. van Rheenen, B.D. Simons, H. Clevers

Nano-imaging of membrane topography affects interpretations in cell biology
2010

K. Jalink, J. van Rheenen

Collagen-based cell migration models in vitro and in vivo
2009

K. Wolf, S. Alexander, V. Schacht, L.M. Coussens, U.H. von Andrian, J. van Rheenen, E. Deryugina, P. Friedl