van Rheenen group
Jacco van Rheenen
Jacco van Rheenen was originally trained in a variety of imaging techniques during his PhD with Dr. Kees Jalink at the Netherlands Cancer Institute. He was among the first to optimize imaging and develop software to quantitatively measure FRET on confocal microscopes. During his PhD in the lab of Dr. Jalink and as postdoc in the lab of Dr. Sonnenberg (Netherlands Cancer Institute) he used several microscopy techniques to study lipid signaling in tumor cells. In order to broader his scales, he obtained a KWF fellowship to do a postdoc in the United States in the lab of Dr. John Condeelis. There he extended his imaging experience by imaging mammary tumors intravitally including two-photon microscopy and became an expert in the field of intravital FRET imaging. In 2008 he was appointed as group leader at the Hubrecht Institute, where he utilizes his imaging techniques to visualize processes that are required for the metastasis of mammary tumor cells in living animals. In 2009, he was awarded a VIDI grant and a research grant from the Dutch Cancer Society. In 2012, he was awarded a research grant from the Association for International Cancer Research (who have now rebranded to Worldwide Cancer Research), and in 2013 a research grant from Netherlands Organisation for Scientific Research. In 2013, he received the Stem Cells Young Investigator Award (see video below). In July 2014 he was appointed professor in Intravital Microscopy at the University Medical Center Utrecht. In 2015, he was awarded an ERC consolidator grant.
Team members van Rheenen
Anko de Graaff
Maria Alieva Krasheninnikova
Jacco van Rheenen
Complication of metastasis, the process in which cells detach from the primary tumor to form new distant tumor sites, is the primary reason why patients die from cancer. Most of our knowledge of tissue homeostasis, tumor initiation, progression and metastasis, and chemotherapy resistance is derived from techniques that draw a static picture of these highly dynamic processes. To study the dynamic aspects of these processes, the van Rheenen group develops state-of-the-art imaging techniques to visualize and study individual cells in real-time in living animals, referred to as intravital microscopy. By these experiments we hope to indetify new drug targets...
Zomer A, Maynard C, Verweij FJ, Kamermans A, Schäfer R, Beerling E, Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SIJ, Wurdinger T, Pegtel DM, van Rheenen J, (2015), In Vivo Imaging Reveals Extracellular Vesicle-Mediated Phenocopying of Metastatic Behavior. Cell, 161(5):1046–1057 Zomer_et_al_extended pdf
Ellenbroek SI, van Rheenen J. (2014) Imaging hallmarks of cancer in living mice. Nature Reviews Cancer, 14(6): 406-18
Ritsma L*, Ellenbroek SI*, Zomer A, Snippert HJ, de Sauvage FJ, Simons BD, Clevers H, van Rheenen J, (2014) Intestinal crypt homeostasis revealed at single-stem-cell level by in vivo live imaging. Nature, 507(7492): 362-5
Ritsma L, Vrisekoop N, van Rheenen (2013) In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique. Nat Commun., 4:2366
Ritsma, L.*, Steller, E.J.A.*, Beerling, E., Loomans, C.J.M., Zomer, A., Gerlach, C., Vrisekoop, N., Seinstra, D., van Gurp, L., Schäfer, R., Raats, D.A., de Graaff, A. Schumacher, T.N., de Koning, E.J.P. Borel Rinkes, I.H., Kranenburg, O. van Rheenen, J. (2012) Intravital Microscopy Through an Abdominal Imaging Window Reveals a Pre-Micrometastasis Stage During Liver Metastasis. Sci Transl Med 4, 158ra145.
Jalink K, van Rheenen, J (2010) Nano-imaging of membrane topography affects interpretations in cell biology. Nat. Meth. 7:486.
Snippert HJ, van der Vlier LG, Sato T, van Es JH, van der Born M, Kroen-Veenboer C, Barker N, Klein AM, van Rheenen J, Simons BD, Clevers H. (2010) Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells, Cell, 143(1): 134-144.
Anoek Zomer, Carrie Maynard, Frederik Johannes Verweij, Alwin Kamermans, Ronny Schäfer, Evelyne Beerling, Raymond Michel Schiffelers, Elzo de Wit, Jordi Berenguer, Saskia Inge Johanna Ellenbroek, Thomas Wurdinger, Dirk Michiel Pegtel, Jacco van Rheenen
Shannon K Hughes, Madeleine J Oudin, Jenny Tadros, Jason Neil, Amanda Del Rosario, Brian A Joughin, Laila Ritsma, Jeff Wyckoff, Eliza Vasile, Robert Eddy, Ulrike Philippar, Alisha Lussiez, John S Condeelis, Jacco van Rheenen, Forest White, Douglas A Lauffenburger, Frank B Gertler
Saskia I J Ellenbroek, Jacco van Rheenen
Laila Ritsma, Saskia I J Ellenbroek, Anoek Zomer, Hugo J Snippert, Frederic J de Sauvage, Benjamin D Simons, Hans Clevers, Jacco van Rheenen
The mechanisms and physiological relevance of glycocalyx degradation in hepatic ischemia/reperfusion injury2014
Rowan F van Golen, Megan J Reiniers, Nienke Vrisekoop, Coert J Zuurbier, Pim B Olthof, Jacco van Rheenen, Thomas M van Gulik, Barry J Parsons, Michal Heger
L. Ritsma, E.J. Steller, S.I. Ellenbroek, O. Kranenburg, I.H. Rinkes, J. van Rheenen
R.D. Fritz, M. Letzelter, A. Reimann, K. Martin, L. Fusco, L. Ritsma, B. Ponsioen, E. Fluri, S. Schulte-Merker, J. van Rheenen, O. Pertz
A. Zomer, S.I. Ellenbroek, L. Ritsma, E. Beerling, N. Vrisekoop, J. van Rheenen
In vivo imaging and histochemistry are combined in the cryosection labelling and intravital microscopy technique2013
L. Ritsma, N. Vrisekoop, J. van Rheenen
A. Janssen, E. Beerling, R. Medema, J. van Rheenen
Tissue-resident memory CD8+ T cells continuously patrol skin epithelia to quickly recognize local antigen2012
S. Ariotti, J.B. Beltman, G. Chodaczek, M.E. Hoekstra, A.E. van Beek, R. Gomez-Eerland, L. Ritsma, J. van Rheenen, A.F. Maree, T. Zal, R.J. de Boer, J.B. Haanen, T.N. Schumacher
Intravital microscopy through an abdominal imaging window reveals a pre-micrometastasis stage during liver metastasis2012
L. Ritsma, E.J. Steller, E. Beerling, C.J. Loomans, A. Zomer, C. Gerlach, N. Vrisekoop, D. Seinstra, L. van Gurp, R. Schafer, D.A. Raats, A. de Graaff, T.N. Schumacher, E. de Koning, I.H. Rinkes, O. Kranenburg, J. van Rheenen
L. Ritsma, B. Ponsioen, J. van Rheenen
If you don't look, you won't see: intravital multiphoton imaging of primary and metastatic breast cancer2012
L. Bonapace, J. Wyckoff, T. Oertner, J. van Rheenen, T. Junt, M. Bentires-Alj
A.W.M. Zomer, E. Beerling, E.J. Vlug, J. van Rheenen
E.J. Steller, L. Ritsma, D.A. Raats, F.J. Hoogwater, B.L. Emmink, K.M. Govaert, J. Laoukili, I.H. Rinkes, J. van Rheenen, O. Kranenburg
E. Beerling, L. Ritsma, N. Vrisekoop, P.W. Derksen, J. van Rheenen
Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells2010
H.J.G. Snippert, L.G. van der Flier, T. Sato, J.H. van Es, M.M.W. van den Born, C. Kroon-Veenboer, N. Barker, A.M. Klein, J. van Rheenen, B.D. Simons, H. Clevers
K. Jalink, J. van Rheenen
K. Wolf, S. Alexander, V. Schacht, L.M. Coussens, U.H. von Andrian, J. van Rheenen, E. Deryugina, P. Friedl