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Hubrecht Imaging Center

Imaging is getting increasingly important for biomedical research, including the research at the Hubrecht Institute. In order to facilitate the fast growing demand on imaging tools, in 2009 the ‘Hubrecht Imaging Center (HIC)’ was founded. The last few years there are a number of advanced microscopes acquired by the institute, which are now all part of the center (see Microscopes). Current imaging at the HIC ranges from simple phase-contrast imaging of cells to high-resolution imaging of living tissue. Advanced systems that are available at the HIC are confocal, two-photon, TIRF, FRAP, and FRET (including FLIM) microscopy. Not only modern, advanced microscopes are part of the imaging center but also some historic microscopes have been retained. In the stairwell next to the main entrance some of these historic microscopes are displayed in a showcase. This gives a nice overview of the history of imaging at the Hubrecht institute.


Staff of the HIC
The HIC is headed by the microscopy manager Anko de Graaff as part of the groups of Johan de Rooij and Jacco van Rheenen. New users should contact the staff in order to get a full instruction before they are allowed to use the microscopes. The staff gives extra support and advice for setting up and performing complex imaging experiments when needed. Furthermore, all microscopes are tested regularly to guarantee high performance. Through the periodic testing and optimization of all systems, training of new researchers and providing support to advanced imaging experiments, the HIC ensures optimal use of the advanced imaging equipment at the Hubrecht Institute.


Microscopes

Leica DM6000 Fluorescence microscope
Applications: fluorescence and DIC imaging

Leica AF7000 High Speed Fluorescence microscope
Applications: fluorescence, DIC and Phase Contrast imaging, high speed “live cell” imaging

Leica SPE Confocal Microscope
Applications: multi-channel 4-D acquisition, spectral detection

Leica SPE 'live cell imaging' Confocal Microscope
Applications: multi-channel 4-D acquisition, spectral detection

Leica SP2 Confocal Microscope
Applications: muulti-channel 4-D acquisition, spectral detection, FRET, FRAP

Leica SP5 Intravital Imaging Microscope
Applications: multi-channel 4-D acquisition, spectral detection, FRET, FRAP, FLIM, intravital imaging, high speed imaging

Leica SP5 Confocal/Multiphoton Microscope
Applications: multi-channel 4-D acquisition, spectral detection, FRET, FRAP, intravital imaging

NIKON TIRF microscope
Applications: TIRF imaging, Epi-fluorescence and DIC imaging

PerkinElmer Ultraview VoX spinning disk microscope
Applications: multi-channel 4-D acquisition, high speed live cell imaging, FRET, FRAP


Contact:

Anko de Graaff
Microscopy manager
T +31 (0)30 212 18 43
a.graaff@hubrecht.eu

Johan de Rooij
Group leader
T +31 (0)30 212 19 60
j.derooij@hubrecht.eu

Jacco van Rheenen
Group leader
T +31 (0)30 212 19 05
j.vanrheenen@hubrecht.eu

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

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